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Addgene inc pmd2g addgene
Pmd2g Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd2g+addgene/pMD2%2EG+(Plasmid+%2312259)/pm41865372-468-142-143
Average 98 stars, based on 14643 article reviews

pmd2g addgene - by Bioz Stars, 2026-09

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Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PET28A-His-UBE2L6 S26A This paper N/A PET28A-His-UBE2L6 S26D This paper N/A PET28A-His-ISG15 This paper N/A pcDNA3.1-4xFLAG-cGAS WT This paper N/A pcDNA3.1-4xFLAG-cGAS DBD This paper N/A pcDNA3.1-4xFLAG-cGAS NTASE This paper N/A pcDNA3.1-4xFLAG-cGAS NTASE+CTD This paper N/A pcDNA3.1-4xFLAG-cGAS CTD This paper N/A pcDNA3.1-4xFLAG-cGAS K171R This paper N/A pcDNA3.1-4xFLAG-cGAS K219R This paper N/A pcDNA3.1-4xFLAG-cGAS K421R This paper N/A pcDNA3.1-4xFLAG-cGAS 3KR (171,219,421) This paper N/A pLKO.1 mRnf19a-1 This paper N/A pLKO.1 mRnf19a-2 This paper N/A pLKO.1 mRnf19b-1 This paper N/A pLKO.1 mRnf19b-2 This paper N/A pLKO.1 mUbe2l6-1 This paper N/A pLKO.1 mUbe2l6-2 This paper N/A pLKO.1 hUBE2L3-1 This paper N/A pLKO.1 hUBE2L3-2 This paper N/A pLKO.1 hUBE2E2-1 This paper N/A pLKO.1 hUBE2E2-2 This paper N/A pLKO.1 hUBE2L6-1 This paper N/A pLKO.1 hUBE2L6-2 This paper N/A FLAG-RIG-I This paper N/A FLAG-MAVS This Study N/A pcDNA3.1-STING-FLAG This paper N/A pcDNA3.1-4xFLAG-TBK1 This paper N/A PET28A-EGFP-RNF19A (1–454) This paper N/A PET28A-EGFP-RNF19A (1–365) This paper N/A PET28A-EGFP-RNF19A (366–454) This paper N/A PET28A-EGFP-RNF19A (366–454, GXXXG Mut) This paper N/A pcDNA3.1-HA-Ub WT This paper N/A pcDNA3.1-HA-Ub R72A This paper N/A pcDNA3.1-HA-Ub K33 Only This paper N/A pcDNA3.1-HA-Ub K6R This paper N/A pcDNA3.1-HA-Ub K11R This paper N/A pcDNA3.1-HA-Ub K27R This paper N/A pcDNA3.1-HA-Ub K29R This paper N/A pcDNA3.1-HA-Ub K33R This paper N/A pcDNA3.1-HA-Ub K48R This paper N/A pcDNA3.1-HA-Ub K63R This paper N/A pcDNA3.1-HA-ISG15 This paper N/A PET28A-HIS-ISG15 This paper N/A lentiGuide-Puro-sgRNA Addgene Cat# 52963 lentiCas9-Blast Addgene Cat# 52962 PSPAX2 Addgene Cat# 12260 PMD2G Addgene Cat# 12259 (Continued on next page) Cell Reports 44, 116323, October 28, 2025 21

Article Title: Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 T cell exhaustion and anti-PD-1 resistance
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER human Tim3 primer 2 Reverse primer (50-30): CTGCTACTGCATTTGCCAATCCTG Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Forward primer (50-30): TGGTGGCACATTTTATGTTGAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Reverse primer (50-30): AAGTTCTGGACCATTAGCAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Forward primer (50-30): GACTCTAGGGTTCTAGCCCAC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Reverse primer (50-30): GGAGACTGCAAACGTGAAACAT Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Forward primer (50-30): TGAGTATTGCCAAGTTTGAGGTCA Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Reverse primer (50-30): CGGCAACAGCTGGTGGAC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Forward primer (50-30): AGTGACAAGCCTGTAGCCC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Reverse primer (50-30): GAGGTTGACTTTCTCCTGGTAT Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Forward primer (50-30): GGAGCAGCTGTTGATGGACCTAC Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Reverse primer (50-30): AATCCAGAACATGCCGCAGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Forward primer (50-30): AGTTCCAGCACATTTTGCGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Reverse primer (50-30): TCATCCTCCGTGAGTTCTCCA Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Forward primer (50-30): AGTTGCGTTACACCCTTTC Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Reverse primer (50-30): CCTTCACCGTTCCAGTTT Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 sense: TGGGAGTCTACACACTAGATA Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 anti-sense: TATCTAGTGTGTAGACTCCCA Beijing Tsingke Biotech Co., Ltd. N/A sgCEACAM-1: TTCACGGGGCAAGCATACAG Beijing Tsingke Biotech Co., Ltd. N/A Recombinant DNA pT3/NICD1 Addgene (Gift from Xin Chen) #86500 pCMV(CAT)T7-SB100 Addgene (Gift from Zsuzsanna Izsvak) #34879 pT3-c-Myc Addgene (Gift from Xin Chen) #92046 pMXs-IRES-GFP Gift from Hongyan Wang N/A MSCV-pU6-(BbsI)-CcdB-(BbsI)Pgk-Puro-T2A-BFP Addgene cat#86457 psPAX2 Addgene cat#12260 pMD2G Addgene cat#12259 pHelper MiaoLingBio cat#P0243 pAAV-DJ MiaoLingBio cat#P2924 pCL-Eco Addgene cat#12371 pT3-AKT-Fluc This paper N/A pT3-AKT-Fluc-OVAp This paper N/A pT3-sTim-3 This paper N/A (Continued on next page) e3 Cell Reports Medicine 5, 101686, August 20, 2024 Article ll OPEN ACCESS

Article Title: Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 + T cell exhaustion and anti-PD-1 resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER human Tim3 primer 2 Reverse primer (50-30): CTGCTACTGCATTTGCCAATCCTG Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Forward primer (50-30): TGGTGGCACATTTTATGTTGAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Reverse primer (50-30): AAGTTCTGGACCATTAGCAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Forward primer (50-30): GACTCTAGGGTTCTAGCCCAC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Reverse primer (50-30): GGAGACTGCAAACGTGAAACAT Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Forward primer (50-30): TGAGTATTGCCAAGTTTGAGGTCA Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Reverse primer (50-30): CGGCAACAGCTGGTGGAC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Forward primer (50-30): AGTGACAAGCCTGTAGCCC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Reverse primer (50-30): GAGGTTGACTTTCTCCTGGTAT Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Forward primer (50-30): GGAGCAGCTGTTGATGGACCTAC Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Reverse primer (50-30): AATCCAGAACATGCCGCAGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Forward primer (50-30): AGTTCCAGCACATTTTGCGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Reverse primer (50-30): TCATCCTCCGTGAGTTCTCCA Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Forward primer (50-30): AGTTGCGTTACACCCTTTC Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Reverse primer (50-30): CCTTCACCGTTCCAGTTT Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 sense: TGGGAGTCTACACACTAGATA Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 anti-sense: TATCTAGTGTGTAGACTCCCA Beijing Tsingke Biotech Co., Ltd. N/A sgCEACAM-1: TTCACGGGGCAAGCATACAG Beijing Tsingke Biotech Co., Ltd. N/A Recombinant DNA pT3/NICD1 Addgene (Gift from Xin Chen) #86500 pCMV(CAT)T7-SB100 Addgene (Gift from Zsuzsanna Izsvak) #34879 pT3-c-Myc Addgene (Gift from Xin Chen) #92046 pMXs-IRES-GFP Gift from Hongyan Wang N/A MSCV-pU6-(BbsI)-CcdB-(BbsI)Pgk-Puro-T2A-BFP Addgene cat#86457 psPAX2 Addgene cat#12260 pMD2G Addgene cat#12259 pHelper MiaoLingBio cat#P0243 pAAV-DJ MiaoLingBio cat#P2924 pCL-Eco Addgene cat#12371 pT3-AKT-Fluc This paper N/A pT3-AKT-Fluc-OVAp This paper N/A pT3-sTim-3 This paper N/A (Continued on next page) e3 Cell Reports Medicine 5, 101686, August 20, 2024

Article Title: Deubiquitinating enzyme MINDY2 regulates TNF-α-induced cell death by targeting RIPK1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER qPCR primer for Mindy2 F: CAGGGCTGCACCTCTATGC This paper N/A qPCR primer for Mindy2 R: CGGGAAAGCGTTCTTTGGAG This paper N/A qPCR primer for Gapdh F: AGGTCGGTGTGAACGGATTTG This paper N/A qPCR primer for Gapdh R: GGGGTCGTTGATGGCAACA This paper N/A shRNA for Hoip F1: CAGAGAAACAACGCCAAGATA This paper N/A shRNA for Hoip R1: TATCTTGGCGTTGTTTCTCTG This paper N/A shRNA for Hoip F2: CTCACTGATGACGCTCAGTTA This paper N/A shRNA for Hoip R2: TAACTGAGCGTCATCAGTGAG This paper N/A shRNA for Mindy1 F1: GACTTTGAGTATACGCCGGAA This paper N/A shRNA for Mindy1 R1: TTCCGGCGTATACTCAAAGTC This paper N/A shRNA for Mindy1 F2: GCATCTTTGACTTGCTGGGAA This paper N/A shRNA for Mindy1 R2: TTCCCAGCAAGTCAAAGATGC This paper N/A shRNA for Mindy2 F1: ATCAGAAATTCAACGTGTAAA This paper N/A shRNA for Mindy2 R1: TTTACACGTTGAATTTCTGAT This paper N/A shRNA for Mindy2 F2: AGTCGGTGTATCACATCAAAT This paper N/A shRNA for Mindy2 R2: ATTTGATGTGATACACCGACT This paper N/A Recombinant DNA PxpAx2 Addgene Cat#12260 PMD2g Addgene Cat#12259 pLKO.1 Addgene Cat#158646 pBOBI MYC-MINDY2 This paper N/A pBOBI HA-MINDY2 This paper N/A pBOBI FLAG-MINDY2 This paper N/A pBOBI MYC-Ripk1 This paper N/A pBOBI HA-Ripk1 This paper N/A pCDNA HA-K48-Ub This paper N/A pCDNA HA-K63-Ub This paper N/A pCDNA HA-Ub This paper N/A Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ GraphPad Prism 8 GraphPad Software http://www.graphpad.com/ Other Protease Inhibitor Cocktail TargetMol Cat#C0001 CCK-8 (K1018, APExBIO) APExBIO Cat#K1018 Cell Reports 45, 117134, April 28, 2026 15

Software:

Article Title: N 6 -methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays PAGE Gel Fast Preparation Kit (10%) EpiZyme Cat# PG112 PAGE Gel Fast Preparation Kit (12.5%) EpiZyme Cat# PG113 Cell Apoptosis TUNEL Kit KeyGene Biotech Cat# KGA7061 Homogentisate ELISA Kit MEIMIAN Cat# MM-926091O2 Tyrosine ELISA kit Fantai BioTech Cat#FT-J3772Q OxyBlot Protein Oxidation Detection Kit Merck Millipore Cat# S7150 Nuclear and cytoplasmic protein extraction Kit Beyotime Cat# P0027 Cell Counting Kit-8 Dojindo Cat# CK04 PierceTM BCA Protein Assay Kit ThermoFisher Cat# 23227 Hiscript III 1st Strand cDNA Synthesis Kit Vazyme Cat# R312-02 SYBR Green Pro Taq HS qPCR kit Accurate Biology Cat# AG11740 Immunohistochemistry Kit DAKO Cat# GK500705 H&E staining Kit Solarbio Cat# G1120 Chemistar High-sig ECL Western blotting substrate Tanon Cat#180-5001 Immunoprecipitation Kit with Protein A + G Magnetic Beads Beyotime Cat# P2179M Chemiluminescent RNA EMSA Kit Beyotime Cat#GS009 Data and code availability Mass spectrometry of protein ProteomeXchange database (PXD053421) www.proteomexchange.org Mass spectrometry of metabolic flux Metabolights database (MTBLS10895) https://www.ebi.ac.uk/metabolights/ Deposited data Polyribosome-RNA-seq Data This study GSE244431 RIP-seq Data This study GSE244431 Experimental models: Cell lines HuCCT1 Japan Health Sciences Foundation Resources Bank Cat# JCRB0425 RBE Chinese Type Culture Collection Cat# 31800022 4T1 Xiaoming Xie (Sun Yat-sen University Cancer Center) N/A Hep1-6 Shuibin Lin (The First Affiliated Hospital of Sun Yat-sen University) N/A 293T Shuibin Lin (The First Affiliated Hospital of Sun Yat-sen University) N/A HepG2 Shuibin Lin (The First Affiliated Hospital of Sun Yat-sen University) N/A MDA-MB-231 Xiaoming Xie (Sun Yat-sen University Cancer Center) N/A 5-8F Jun Ma (Sun Yat-sen University Cancer Center) N/A Experimental models: Organisms/strains C57BL/6-Zcchc4fl/fl Biocytogen EGE-LYS-0170 Oligonucleotides RT-qPCR primers, siRNAs and sgRNA used This study, Table S1 N/A Recombinant DNA pCDH-CMV-EF1-Puro ZCCHC4-Flag-cDNA human This study N/A (Continued on next page) Cell Reports 44, 115139, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Catalytic dead pCDH-CMV-EF1-Puro ZCCHC4-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro ZCCHC4-F1-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro ZCCHC4-F2-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro ZCCHC4-F3-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro ZCCHC4-F4-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro ZCCHC4-F5-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro ZCCHC4-F6-Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro myc-ZCCHC4Flag-cDNA human This study N/A pCDH-CMV-EF1-Puro eIF3G-HA-cDNA human This study N/A pCDH-CMV-EF1-Puro eIF3D-HA-cDNA human This study N/A pCDH-CMV-EF1-Puro HPD-cDNA human This study N/A pT3-EF1a-HA-Mettl5 mouse This study N/A pT3-EF1a-HA-Hpd mouse This study N/A pT3-EF1a-HA-myr-AKT Addgene Cat# 31789 pT3-EF1a-YapS127A Addgene Cat# 6049 SB transposase Addgene Cat# 24551 LentiCRISPRv2-sgRNA targeting human ZCCHC4 This study N/A psPAX2 Addgene Cat# 12260 pMD2G Addgene Cat# 12259 pETDuetTM-1 Addgene Cat#71146 Software and algorithms Bowtie2 Langmead et al.39 http://bowtie-bio.sourceforge.net FASTQ Chen et al. https://github.com/OpenGene/fastp Trim_galore Babraham Bioinformatics Group https://www.bioinformatics. babraham.ac.uk/projects/trim_galore HISAT2 Sirén et al.40 https://ieeexplore.ieee.org/ document/6698337 IGV Robinson et al.41 https://www.ncbi.nlm.nih.gov/ pmc/articles/PMC3346182/ exomePeak2 Meng et al.42 https://www.sciencedirect.com/ science/article/abs/pii/ S1046202314002308?via%3Dihub MEME Suite National Institutes of Health http://meme-suite.org/ Trim_galore Babraham Bioinformatics Group https://www.bioinformatics. babraham.ac.uk/projects/trim_galore Mascot Perkins et al.49 https://analyticalsciencejournals. onlinelibrary.wiley.com/doi/10.1002/ (SICI)1522-2683(19991201)20:18%3C3 551::AID-ELPS3551%3E3.0.CO;2-2 Percolator Käll et al.50 https://www.nature.com/ articles/nmeth1113 iBAQ Schwanhäusser et al.51 https://www.nature.com/ articles/nature10098 Proteome Discoverer Thermo ScientificTM thermofisher.cn (Continued on next page) 20 Cell Reports 44, 115139, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER InterProScan 5.57–90.0 Global Biodata Coalition https://www.ebi.ac.uk/interpro/ download/interproscan/ Perl Module Grepcpan https://grep.metacpan.org/ ProteoWizard Mallick Lab http://www.proteowizard.org/ R Package (version 3.4.4) N/A http://www.Rproject.org Picard (version 2.5.0) MetaBrainZ Foundation http://broadinstitute.github.io/picard/ GATK (4.1.4.1) McKenna et al.43 https://software.broadinstitute.org/gatk/ GraphPad Prism 8.0 GraphPad Software, Inc. https://www.graphpad.com/ scientificsoftware/prism/ DAVID Bioinformatics Resources v2022q4 Sherman et al.44 https://david.ncifcrf.gov/ ImageJ Schneider et al.45 https://imagej.net/ij/index.html Image Pro Plus Media Cybernetics https://www.epixinc.com/ vision_archive/imagepro.htm

Article Title: V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation.
Article Snippet: Article V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation

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Article Title: Circadian clock features define novel subtypes among breast cancer cells and shape drug sensitivity.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models HCC1143 ATCC CRL-2321 HCC1806 ATCC CRL-2335 HCC1937 ATCC CRL-2336 HCC38 ATCC CRL-2314 HCC70 ATCC CRL-2315 MDAMB468 ATCC HTB-132 BT549 Gift of Prof. Peter Sorger (Harvard) CAL51 Gift of Prof. Peter Sorger (Harvard) HCC1428 Gift of Prof. Peter Sorger (Harvard) MDAMB231 Gift of Prof. Peter Sorger (Harvard) MDAMB436 Gift of Prof. Peter Sorger (Harvard) SUM149PT Gift of Prof. Peter Sorger (Harvard) MCF10A Gift of Prof. Joan Brugge (Harvard) MCF7 Gift of Prof. Joan Brugge (Harvard) T47D Gift of Prof. Joan Brugge (Harvard) U-2 OS Gift of Prof. Achim Kramer (Charité) U-2 OS Cry1-sKO Börding et al, 2019 U-2 OS Cry2-sKO Börding et al, 2019 U-2 OS Cry1/2-dKO Börding et al, 2019 HEK293T Gift of Prof. Galit Lahav (Harvard) Recombinant DNA pAB-mBmal1:LucPuro Gift of Prof. Achim Kramer (Charité) plenti6-mPer2:LucBlas Gift of Prof. Achim Kramer (Charité) psPAX2 Addgene Cat #12260 pMD2G Addgene Cat #12259 gag/pol packaging plasmid Addgene Cat #14887 pRev packaging plasmid Addgene Cat #12253 VSV-G envelope plasmid Addgene Cat #14888 EF1α-mKate2-NLS plasmid Gift of Prof. Galit Lahav (Harvard) 12 Molecular Systems Biology © The Author(s) D ow nloaded from https://w w w .em bopress.org on M arch 1, 2025 from IP 2401:4900:1ce1:f8ae:54ac:d0c0:de67:d2a. .. Reagent/Resource Reference or Source Identifier or Catalog Number Antibodies Not applicable Oligonucleotides and other sequence-based reagents Not applicable Chemicals, Enzymes and other reagents DMEM/F12 Gibco Cat #11320033 RPMI-1640 Gibco Cat #21875091 FluoroBrite DMEM Gibco Cat #A1896701 Horse serum Gibco Cat #26050088 Fetal bovine serum Gibco Cat #10270106 EGF Peprotech Gibco Cat #AF-100-15500UG Hydrocortisone Sigma Cat #H-0888-1g Cholera Toxin Sigma Cat #C-8052-2mg Insulin Sigma Cat #I-1882 PenicillinStreptomycin Gibco Cat #15140122 L-Glutamine Gibco Cat #25030-024 HEPES Gibco Cat #15630056 Lipofectamine 3000 Invitrogen Cat #L3000001 Protamine sulfate Sigma Cat #P3369 PBS Gibco Cat #10010056 Blasticidin Adooq Cat #A14212 Puromycin Gibco Cat #A1113803 Dexamethasone Sigma Cat #D4902 D-Luciferin Abmole Cat #M9053 5-Fluorouracil Sigma Cat #03738-100MG Torin2 Sigma Cat #SML1224-5MG Alpelisib Biozol Cat #TGM-T192110MG Adavosertib Biocat Cat #T2077-5mg-TM Doxorubicin Hölzel Cat #A14403-100 Paclitaxel Hölzel Cat #M1970-50mg Alisertib Hölzel Cat #S1133-5 Olaparib Adooq Cat #A10111 Cisplatin Sigma Cat #232120 Software MATLAB v2021b https://mathworks.com Python Spyder v5.4.5 https://github.com/spyderide/ pyBOAT v0.9.12 Mönke et al, 2020 Anaconda Navigator v2.5.0 https://docs.anaconda.com/ navigator/ Other LumiCycle Actimetrics Incucyte Essen BioScience

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